Phenotypic variability evaluation and genetic variation in F2 intraspecific hybrids of cucumber (Cucumis sativus L.) using retrotransposon-based markers

Abstract. Setiawan AB, Zahidah QA, Kaltsum DN, Purwantoro A. 2023. Phenotypic variability evaluation and genetic variation in F2 intraspecific hybrids of cucumber (Cucumis sativus L.) using retrotransposon-based markers. Biodiversitas 24: 2596-2604. Cucumber has a narrow base compared to other Cucumis species. The the can be improved by crossing two distantly related cultivars from different geographical locations. This study aimed evaluate phenotypic investigate population markers, namely Inter Retrotransposon Amplified Polymorphism (IRAP)andInter-SINE Polymorphism(ISAP). Two parental lines selected 59 genotypes were subjected PCR analysis for DNA profiling. revealed significant diversity fruit weight, diameter, radian, pedicel length, ratio (length-diameter). was successfully detected discriminated IRAP ISAP Both markers showed high heterozygosity ranged 0.4021 0.4997, with an average 0.4649 moderate polymorphic information content 0.3489 0.3929 0.3648. Cluster that grouped into major clusters similarity coefficient 0.67 0.96. These findings imply is highly segregated utilized create new cucumber.